Effects of HCV JFH-1 on gene expression during 48 hour infection of Primary Human Hepatocytes

To investigate the underlying effects of HCV JFH-1 on cellular innate immune response, primary human hepatocytes were infected for 48 hours and analyzed for whole-cell total RNA. Gene expression profiling analysis was subsequently performed using data obtained from Illumina NextSeq 2500 bulk RNA-sequencing of 4 different cell populations. Overall design: Primary human hepatocytes obtained and plated from patient liver resections were either uninfected or infected with Hepatitis C Virus JFH-1 MOI = 1 in duplicate.