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HUVEC mCherry or GPNMB-mChery over expressed, mCherry-IP

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下载链接:
https://www.omicsdi.org/dataset/jpost/PXD013762
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资源简介:
HUVEC were infected retroviral vectors expressing mCherry-GPNMB or mCherry. The cells were lysed with Pierce IP lysis buffer. The lysates after immunoprecipitation using anti-mCherry antibody (ab213511, Abcam) was transferred into a new microcentrifuge tube for “tube gel digestion”, a modified in-gel digestion procedure, to generate dithiothreitol-reduced iodoacetamide-alkylated tryptic peptides for nano-flow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
创建时间:
2022-05-08
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