Gene expression analysis in U251 and U87 cells transduced with NANEP5 vector.

NANEP5 is a heterologus repressor of NANOG, which consist of NANOG homeodomain and HES1 repressor domain. In this study we explore transcriptomes of glioblastoma cells expressing NANEP5 in order to reveal putative mediators of pro-tumorigenic NANOG function. U251 and U87 cells were infected with control (CT) or NANEP5-expressing lentiviral vectors. Total RNA was extracted 10 days after infection using TRIzol Reagent (Ambion) following the manufacturer's protocol. Each condition was independently processed in triplicates and the libraries were prepares using Illimina TruSeq protocols. The samples were sequenced on a Illumina HiSeq 4000 machine with 50bp reads followed by standard analyses.