Genome-wide analysis of PGC-1a-binding in microglia

PGC-1a in microglia protects against ischemia-induced brain damage in mice. The data suggest that microglia-specific PGC-1a play a key role in limiting ischemia-induced brain damage and potently participates in regulating microglial function. To further clarify the mechanism of PGC-1a, we conducted chromatin immunoprecipitation-sequencing (ChIP-Seq) analysis to identify the targets of PGC-1a in microglia from mPGC-1a mice at 24 h after ischemic stroke. KEGG pathway analysis of these genes identified the mitophagy signaling pathway as one of the most highly enriched pathways. Finally, we demonstrated that PGC-1a induces mitophagy by regulating ULK1 expression in an ERRa-dependent manner, thereby reducing neuroinflammatory reactions. Overall design: Primary microglia were isolated from mPGC-1a mice at 24 h after ischemic stroke using the MojoSortTM mouse P2RY12 selection kit (Biolegend, San Diego, CA, USA) according to the manufacturer's protocol. Then the samples were processed to perform ChIP-Seq analysis.