AFLP_data

Binary matrix of nuclear AFLP loci which were generated using a modification of the method of Vos et al. 1995 as applied by Herder et al. 2006. The following 20 MseI/EcoRI primer pairs were used for selective amplification of fragments: AGG*-CTG; ACA*-CAA; ACA*-CTG; ACT*-CAA; AGG*-CTC; ACC*-CTA; ACT*-CAG; ACC*-CAT; AGG*-CTA; ACA*-CAT; ACT*-CTG; ACC*-CAG; ACT*-CTT; AGC*-CTC; AGG*-CAA; AGC*-CAC; AGG*-CTT; AGC*-CAG, ACT*-CAC, ACC*-CTC. Bands were visualized on an AB 3130 sequencer (Applied Biosystems) with size standard ROX 500XL. The Genemapper® v. 4.0. software (Applied Biosystems) was used for automatically scoring peaks between 50 and 499 bases for presence/absence. Three individuals were double genotyped to assess error rates. To correct for standard error of automated sequencers a bin correction was applied to the dataset following Schwarzer et al. 2011.