AFLP_data

Binary matrix of nuclear AFLP loci which were generated using a modification of the method of Vos et al. 1995 as applied by Herder et al. 2006. The following 20 MseI/EcoRI primer pairs were used for selective amplification of fragments: AGG*-CTG; ACA*-CAA; ACA*-CTG; ACT*-CAA; AGG*-CTC; ACC*-CTA; ACT*-CAG; ACC*-CAT; AGG*-CTA; ACA*-CAT; ACT*-CTG; ACC*-CAG; ACT*-CTT; AGC*-CTC; AGG*-CAA; AGC*-CAC; AGG*-CTT; AGC*-CAG, ACT*-CAC, ACC*-CTC. Bands were visualized on an AB 3130 sequencer (Applied Biosystems) with size standard ROX 500XL. The Genemapper® v. 4.0. software (Applied Biosystems) was used for automatically scoring peaks between 50 and 499 bases for presence/absence. Three individuals were double genotyped to assess error rates. To correct for standard error of automated sequencers a bin correction was applied to the dataset following Schwarzer et al. 2011.本数据集为核基因组扩增片段长度多态性（Amplified Fragment Length Polymorphism, AFLP）位点的二值矩阵，其构建基于Vos等人1995年方法的改良版本，并沿用了Herder等人2006年的应用流程。本研究采用以下20对MseI/EcoRI引物组合进行片段的选择性扩增：AGG*-CTG、ACA*-CAA、ACA*-CTG、ACT*-CAA、AGG*-CTC、ACC*-CTA、ACT*-CAG、ACC*-CAT、AGG*-CTA、ACA*-CAT、ACT*-CTG、ACC*-CAG、ACT*-CTT、AGC*-CTC、AGG*-CAA、AGC*-CAC、AGG*-CTT、AGC*-CAG、ACT*-CAC、ACC*-CTC。扩增条带通过AB 3130测序仪（Applied Biosystems，应用生物系统公司）结合ROX 500XL分子量标准进行可视化检测。采用Genemapper® v4.0软件（Applied Biosystems）对50~499碱基区间内的电泳峰进行有无的自动判读。选取3个个体进行重复基因分型以评估实验误差率。为校正自动测序仪的系统误差，本数据集参考Schwarzer等人2011年的方法实施了区间校正（bin correction）。

搜集汇总

背景与挑战

背景概述

该数据集包含坦噶尼喀湖慈鲷鱼类的AFLP（扩增片段长度多态性）基因数据，涉及3312个核AFLP位点和对91个物种的基因分型，用于研究古代杂交事件和物种系统发育。数据以二进制矩阵形式提供，通过20个MseI/EcoRI引物对生成，并经过自动化测序仪和软件处理，支持坦噶尼喀湖慈鲷鱼类'熔炉'起源假说的验证。