Genomic analyses of the scorpion mud turtle Kinosternon scorpioides in continental and insular Colombia

The turtle genus Kinosternon is widespread with at least 25 species distributed from Mexico to northern Argentina. The taxonomy of this genus is controversial and requires a full revision using both morphological and molecular approaches. In this study, we did a genomic analysis on the species Kinosternon scorpioides distributed in insular and continental Colombia in order to define conservation units. Total DNA was extracted from 24 tissue samples and RADseq genotyping analysis was done. In addition, the intron R35 was amplified and sequenced for a subset of samples. A total of 35,507 SNPs combined with 1,047 bp of the intron were used for spatiotemporal colonization patterns reconstruction and phylogenetic analyses. In addition, the SNPs were used for population structure inferences and allele frequency-based analyses.   According to the long-term reciprocal monophyly, significant differences in allele frequencies (Fst = 0.37 - 0.8), and evidence of reproductive isolation (no admixtur...