一种单宁酸的高效液相色谱分析方法

本技术提供了一种单宁酸的高效液相色谱
分析方法，即首先将单宁酸用有机溶剂或水溶解
制成单宁酸待测样品溶液，利用HPLC对其进行洗
脱得到单宁酸HPLC指纹图谱；利用LC-MS对单宁
酸中不同组分进行定性分析；根据对单宁酸中不
同聚体多酚化合物的保留时间及其聚合度相关
性分析建立不同组分的保留时间与其聚合度之
间的数学模型；将各组分的保留时间代入该数学
模型可计算出该化合物的聚合度(分子量)；根据
不同组分的色谱峰面积利用面积归一化法计算
单宁酸不同组分的含量。该方法操作简单、准确，灵敏度高，分析时间短。

This technology provides a high performance liquid chromatography (HPLC) analysis method for tannic acid. Specifically, first dissolve tannic acid with an organic solvent or water to prepare the sample solution to be tested. Elute the solution using HPLC to obtain the HPLC fingerprint chromatogram of tannic acid, and conduct qualitative analysis on different components in tannic acid via liquid chromatography-mass spectrometry (LC-MS). Establish a mathematical model between the retention time and degree of polymerization of each component by analyzing the correlation between the retention time and degree of polymerization of different oligomeric polyphenol compounds in tannic acid. Substitute the retention time of each component into this mathematical model to calculate the degree of polymerization (molecular weight) of the corresponding compound. Calculate the content of each component of tannic acid using the area normalization method based on the chromatographic peak areas of different components. This method features simple operation, high accuracy, high sensitivity and short analysis time.

搜集汇总

背景与挑战

背景概述

该数据集描述了一种用于分析单宁酸的高效液相色谱（HPLC）方法，结合LC-MS定性分析，通过建立保留时间与聚合度的数学模型来计算单宁酸各组分的聚合度和含量，具有操作简单、准确度高和快速分析的特点。数据集属于“人工林非木质资源全产业链增值增效技术集成与示范”项目，旨在支持林业非木质资源的技术开发，数据以文档格式提供，适用于植物学和化学分析领域的研究。