RNA-seq of long-term hematopoietic stem cells and myeloid progenitors of control mice or mice conditionally deficient for Trp53 or Elp3 or both genes in the hematopoietic system

Aim of the experiment was to compare the gene expression responses of long-term hematopoietic stem cells (HSCs) and myeloid-restricted progenitors (MyPs) to the hematopoietic cell-restricted deletion of the catalytic subunit Elp3 of the Elongator complex. Since Elp3 deletion resulted in activation of the p53 pathway, we also studied the transcriptome of MyPs deficient for Trp53 or for both Elp3 and Trp53. The Elp3fl/fl strain was generated in house and first described in (DOI: 10.1084/jem.20142288). The Trp53 strain was first described in (DOI: 10.1101/gad.14.8.994) and was purchased from the Jackson Laboratory. Littermates of 8-12 weeks old were used in all experiments.本实验的目的是比较长期造血干细胞（long-term hematopoietic stem cells, HSCs）与髓系限制性祖细胞（myeloid-restricted progenitors, MyPs）在造血细胞特异性敲除延伸复合物（Elongator complex）催化亚基Elp3后的基因表达应答差异。鉴于Elp3敲除会激活p53通路，本研究还对Trp53缺陷或同时缺陷Elp3与Trp53的髓系限制性祖细胞的转录组进行了分析。Elp3fl/fl品系由本实验室构建，并首次在文献（DOI: 10.1084/jem.20142288）中报道。Trp53品系首次见于文献（DOI: 10.1101/gad.14.8.994），并购自杰克逊实验室（Jackson Laboratory）。所有实验均采用8-12周龄的同窝仔鼠。