GlycoRNA identification by rPAL enrichment from acute myeloid leukemia cell lines and derived extracellular vehicles

These data are used to identify the classes of glycoRNAs from AML cell lines and their derived EVs. GlycoRNAs were enriched from KG1a and HL60 cells, and their derived large/small EVs, using the corresponding total RNA as the input controls. Overall design: Total RNAs were extracted from KG1a and HL60 cells, and their derived large and small EVs using RNAzol. GlyocRNA (sialyloglycoRNAs) were labeled with biotin using periodate oxidation and aldehyde ligation (rPAL) and enricehd, purfied and subjected to small RNA sequencing with their corresponsing total RNAs as the input controls. Small RNA libraries were prepared by MGIEasy Small RNA Library Prep Kit (BGI, Shenzhen, China), followed by sequencing on DNBSEQ-G400 platform by BGI-Shenzhen (China).