RNA-seq of WTAP knockdown human pluripotent stem cells during the exit from pluripotency induced by Activin/Nodal inhibition

This experiment was designed to monitor the gene expression changes induced by the knockdown of WTAP in human pluripotent stem cells (hPSCs) during the exit from pluripotency induced by the inhibition of Activin/Nodal signalling. hPSCs carrying a tetracycline-inducible short hairpin RNA (shRNA) against WTAP were cultured in control conditions or in presence of tetracycline (TET) to drive gene knockdown. Cells were monitored by RNA-seq in the pluripotent state (culture conditions containing Activin), or at various time points after inhibition of Activin/Nodal signalling with the receptor antagonist SB-431542 (SB). Samples were collected after 2h, 8h and 32h.