Characterizing the combined effects of cytochrome P450 missense variation within star allele definitions

<b>Supplementary Figure 1. Comparison of Fold-X computed (∆∆</b><b>G</b><b>) and ENCoM-computed (∆∆</b><b>S</b><b>vib</b><b>).</b> Each haplotype is represented by a maroon square and their defining missense variants are represented by circles. Figure points that fall within the highlighted region are expected to have neutral effects on protein stability [1]. ∆∆G predicts most of the variants and haplotypes as destabilising to the protein encoded protein structures. Both ∆∆G and ∆∆S_vib seem to exhibit a destabilising bias for both haplotype and variants. However, ∆∆S_vib predicted more haplotypes and variants to have neutral effects on protein stability than ∆∆G (see also Figure 3A).<b>Supplementary Table 1. </b>This table presents all 261 missense variants analysed in this study, together with their FoldX and ENCoM-derived energy scores. A combined ∆∆G (combination of ENCoM and FoldX) is presented, from which a prediction of the effect of each variant is made based on the threshold (-0.5 &lt; combined ∆∆G &lt; 0.5) [1]. Predictions from commonly used Ensembl Variant Effect Predictor (VEP; https://www.ensembl.org/ToolsVEP) [2] plugins as well as a consensus (2 from 3 rule) has been included for comparison with combined ∆∆G predictions<b>Supplementary Table 2. CYP star allele/haplotype NMA analysis.</b><i> </i>This table presents 92 CYP star alleles together with the defining missense variants for each star allele. Moreover, a CPIC functional classification is included together with the NMA-based combined ∆∆G analysis.<b>Supplementary Table 3. SWAAT analysis of CYP missense variant structural consequence.</b>