Dynamic microtubules produce an asymmetric E-cadherin–Bazooka complex to maintain segment boundaries

A time-lapse movie of FRAP of Baz-GFP co-expressed with Shi-DN expressed using en::Gal4 in Drosophila embryonic epidermis at stage 15. In each embryo several circular regions of 1 µm radius were photobleached at junctions. Photobleaching was performed with 12 scans at 8 µs/pixel at 100% 488 laser power, resulting in the reduction of E-cad-GFP signal by 60-80%. A stack of 6 z-sections spaced by 0.38 µm was imaged just before photobleaching, and immediately after photobleaching, and then at 20 sec intervals, for 15 min in total. Embryo 2. Region 1