IFNb/LPS activates convergence of inflammatory gene expression patterns between adipocytes and myeloid cells.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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We examined the transciptomic changes to primary adipocytes resulting from exposure to IFNb and/or LPS, in order to define the mechanisms driving adipocyte inflammatory capacity and resulting transcriptomic convergence with iinflammatory myeloid cells.Inguinal adipose tissue was obtained from 8 weeks old aged male C57BL/6 mice and stromal vascular fraction was maximally differentiated into primary adipocytes. Cultured cells were treated with IFNb (200U/ml) or saline for 3 hours and thereafter with LPS (100ng/ml) or saline for 4 hours and then submitted for RNA-sequencing.Results: We mapped approximately 20 million reads per sample to the mm10 genome, and analyzed all transcripts with >3 reads in 100% of at least one experimental condition.
提供机构：
Cincinnati Children's Hospital Medical Center
创建时间：
2022-02-20
