IFNb/LPS activates convergence of inflammatory gene expression patterns between adipocytes and myeloid cells.

We examined the transciptomic changes to primary adipocytes resulting from exposure to IFNb and/or LPS, in order to define the mechanisms driving adipocyte inflammatory capacity and resulting transcriptomic convergence with iinflammatory myeloid cells.Inguinal adipose tissue was obtained from 8 weeks old aged male C57BL/6 mice and stromal vascular fraction was maximally differentiated into primary adipocytes. Cultured cells were treated with IFNb (200U/ml) or saline for 3 hours and thereafter with LPS (100ng/ml) or saline for 4 hours and then submitted for RNA-sequencing.Results: We mapped approximately 20 million reads per sample to the mm10 genome, and analyzed all transcripts with >3 reads in 100% of at least one experimental condition.