Compare the knock-down effects of SLC2A11-MIF and two parent genes in Hela cell line

Total RNA were extracted from five knocking down treated Hela cells using siRNA targeting fusion gene SLCC2A11-MIF and parental genes To investigate the function of fusion gene and parental gene, we designed siRNAs specifically knocking down the fusion RNA SLC2A11-MIF and parental gene SLC2A11 respectively. Totally we have five samples. One sample is control, two siRNAs target parental genes and other two siRNAs target fusion genes. After 48hrs siRNA treatment, the RNAs were extracted from cells, and followed by RNA microarray. one siRNA negative control, four siRNAs against SLC2A11, MIF, and SLCA211-MIF(two siRNAs)