Supplementary Table 1 - Small extracellular vesicles from iPSC-derived mesenchymal stem cells ameliorate tendinopathy pain by inhibiting mast cell activation

<b>Supplementary
Information</b>

Abstract
Aims: This study
aimed to explore the effect of iMSC-sEVs on acute pain and investigate the
underlying mechanisms.
Materials &amp;
Methods: The pathology of tendon was accessed by H&amp;E staining,
immunohistochemical and immunofluorescent staining. The pain degree was
measured by pain-related behaviors. In vitro, we performed -Hexosaminidase
release assay, RT-qPCR, Toluidine blue staining, ELISA and RNA-seq.
Results: iMSC-sEVs
effectively alleviated acute pain in tendinopathy as well as inhibited
activated mast cell infiltration and interactions with nerve fibers in vivo.
In vitro, iMSC-sEVs reduced the degranulation of mast cells, the expression
of proinflammatory cytokines and genes involved in the HIF-1 signaling
pathway.
Conclusions: This
study demonstrated iMSC-sEVs relieved tendinopathy-related pain through
inhibiting mast cells activation via the HIF-1 signaling pathway.