Construction the adult Drosophila cell atlas at single cell resolution

Single-cell mRNA sequencing (mRNA-seq) technologies are reshaping the current cell-type classification system. In previous studies, we built the mouse cell atlas (MCA) and human cell landscape (HCL) to catalog all cell types by collecting scRNA-seq data. However, systematically study for zebrafish (Danio rerio) and fruit fly (Drosophila melanogaster) are still lacking. Here, we construct the zebrafish and Drosophila cell atlas with Microwell-seq protocols, which provides valuable resources for characterization of diverse cell populations of zebrafish and Drosophila, and studying difference between vertebrates and Invertebrates at single cell level. Over 270,000 cells derived from Drosophila whole-body single-cell dissociation and profiling at 11 day (D11) and 16 day (D16) after laying eggs (nearly 25-30 fruit flies per timepoint).