Helicobacter pylori metal homeostasis: Fur and NikR regulons defined by RNA-seq and ChIP-seq

Helicobacter pylori is a human pathogen surviving in the prohibitive gastric environment where it can cause cancer. The ability to counteract environmental stresses and host responses is a key feature for persistence and depends on the rapid and fine-tuned expression of the genetic repertoire orchestrated by a shallow Transcriptional Regulatory Network. Metal-dependent regulators NikR and Fur are key hubs in this network; they directly control metal ion homeostasis and indirectly impact on several processes involved in H. pylori virulence and survival.In this work we combined ChIP- and RNA-sequencing to gain a comprehensive view of Fur and NikR regulomes.