Organ Scaffold Characterizations Using Intravital Microscopy Repository

<b>Abstract</b>This study contains intravital microscopy (IVM) data used to characterize whole organ scaffolds created using decellularization. Decellularization is a process that creates a cell-free collagen-based matrix from native organs, which can be used as scaffolds for regenerative medicine applications. This data set contains <i>in vivo</i>assays that evaluated the effectiveness of the decellularization process and structural and functional integrity of the acellular nephron in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, structural and functional integrities of various decellularized nephron segments, and velocity within the microcirculation were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n=4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). OIF, OIB, FV Multi-Tiff formatted files illustrate IVM measurement processes and are made publicly available in a data repository. Such data is intended to support scientific reproducibility, complement future renal studies, and extend the use of this powerful imaging application to analyze other organ scaffold systems.