Transcriptional profiling of WT Tregs and Tregs sorted from K14cre;Cdh1F/F;Trp53F/F mice bearing 225mm2 mammary tumors from blood, spleen, lungs, TDLNs, tumor and healthy mammary gland [Treg]

We studied the impact of mammary tumorigenesis on Tregs in tumors and distant organs (CD3+CD4+CD25+). Here we generated RNAseq data from sorted Tregs (CD3+CD4+CD25+) from WT and K14cre;Cdh1F/F;Trp53F/F mice bearing 225mm2 mammary tumors from blood, spleen, lungs, TDLNs, tumor and healthy mammary gland Overall design: In this study, gene expression profiles of Tregs sorted from K14cre;Cdh1F/F;Trp53F/F mice bearing 225mm2 mammary tumors, or non-tumor bearing WT littermates from blood, spleen, lungs, TDLNs, tumor and healthy mammary gland were analysed by RNAsequencing. Each WT sample is a sorted pool of n=3 WT mice. We generated 3 biological replicates for spleen/lung/blood/tumor/TDLN, and 4 for healthy mammary gland. Samples were grouped according to tissue and tumor status. Comparisons were made between Tregs derived from KEP and WT mice.