Nfkbiz−/− mouse T cells fail to generate Th17 cells in vitro.

(A–D) Expression of IL-17A protein or Il-17a mRNA (A, B) and of the Th17-related mRNAs Ccr6 and Il-17f (C, D) in CD4+ T cells from Nfkbiz+/+ and Nfkbiz−/− mice, cultured for 48 h under Th0 or Th17 conditions. (E) Diagram of Il-17a and Il-17f gene conservation. Red-arrows indicate the Il-17a promoter, the Il-17f promoter, and the CNS 1, CNS 2, and CNS 3 regions. (F) ChIP analysis of H3K27Ac. Cells were cultured under Th0 or Th17 conditions for 48 h. Data shown are from one experiment that was representative at three independent experiments. (A–D) Data shown represent mean ± S.E. (n = 3). Paired data were evaluated using the Student's t test. *pp