Gene expression profile at single cell level of FACS sorted CD8+ T cells from subcutaneous MB49 tumors from male and female mice

Tumor-infiltrating CD8+ T cells are phenotypically heterogeneous with varying degrees of functional defects. We used single cell RNA sequencing (scRNA-seq) to analyze their sex differences. Tumor-infiltrating CD8+ T cells in male and female mice were isolated by Fluorescence-Activated Cell Sorting (FACS) from MB49 s.q. tumors on Day 10 post inoculation according to their CD3 and CD8 expression and analyzed using scRNA-seq