Additional file 1: Figure S1. of The combined effect of PDX1, epidermal growth factor and poly-L-ornithine on human amnion epithelial cells’ differentiation

Comparison of transduction efficiency of various adenoviral titres. (a) hAECs were transduced with adenovirus harbouring an mPdx1 vector at various MOIs (multiplicity of infection). Cells were stained with an mPdx1-specific antibody (Texas-Red conjugate) at 24 h and 48 h post infection to determine the transduction efficiency. Nuclei were counter stained with DAPI (blue). (b) Calculation of transduction efficiency from two different microscopic fields of cells 24 h after transduction with 50 MOI of the mPdx1-harbouring adenovirus. Cells were viewed using the 10X objective of an Olympus inverted fluorescence microscope. Purple nuclei are those that are stained by both DAPI and Texas-Red conjugated secondary antibody. (ZIP 1201 kb)