Dynamic N6-Methyladenosine profiling during human lung progenitor cell differentiation from iPSCs

Dynamic RNA N6-Methyladenosine (m6A) modifications are of essential significance in governing RNA synthesis and metabolism during embryonic development. Nevertheless, the comprehensive profile and functional mechanisms of m6A in the early phase of embryonic lung development remain largely elusive. To delineate the dynamic mapping of m6A and investigate the underlying regulatory mechanisms during the process of embryonic lung differentiation, we carried out picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) through out the stages of endoderm lung progenitor differentiation derived from human induced pluripotent stem cells (iPSCs). We first induced the generation of definitive endoderm (DE), anterior foregut endoderm (AFE), and lung progenitor (LP) cells from induced pluripotent stem cells (iPSCs). Subsequently, we carried out picogram-scale m6A RNA immunoprecipitation and high-throughput sequencing (picoMeRIP–seq) with the aim of simultaneously capturing the m6A epitranscriptome and transcriptome throughout the entire differentiation process. *************************************************************** The raw and processed data for this Series are available in the China National Center for Bioinformation (https://ngdc.cncb.ac.cn/) under accession number HRA012195 in compliance with the Chinese government's regulations on human genetic information. ***************************************************************