To investigate whether recover DNMT3A expression permitted adult beta cell of βRapKO establishing proper transcriptome. To investigate whether recover DNMT3A expression permitted adult beta cell of βRapKO establishing proper transcriptome

Murine islets were isolated from 8-week-old WT and βRapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80°C for preparation for RNA sequencing. Overall design: Transcriptome of three groups: WT, βRapKO, βRapKO+LvDnmt3a