Surface CD52, CD84 and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors [bulk RNA-seq]

In this study, we reported the identification of a specific gene signature expressed by mPMN-MDSCs from NSCLC/HNC patients and G-CSF-treated donor (GDs) by bulk-RNA-seq that clearly reflects their metabolic/functional reprogramming. ScRNA-seq experiments further confirmed that the mPMN-MDSC gene signature is significantly enriched in both mNDNs/mLDNs from GDs. Overall design: CD66b+CD10+CD11b+CD16+ GD-mLDNs, GD-mNDNs and control HD-mNDNs were isolated from the peripheral blood (PB) of GDs or healthy donors (HDs) by flow cytometry sorting. CD66b+CD10+CD11b+CD16+ mPMN-MDSCs and autologous mNDNs from NSCLC or HNC patients, as well as control HD-mNDNs, were isolated from the PB by cell sorting via CD66b+ beads or by flow cytometry sorting. CD66b+CD10-CD11b+CD16+ band cells (BCs), CD66b+CD10-CD11b+CD16+/dim metamyelocytes (MMs), CD66b+CD10-CD11bdimCD16- myelocytes (MYs) and CD66b+CD10-CD11b-CD16- promyelocytes (PMs) from either the PB of GDs by flow cytometry sorting. Total RNA was then extracted and subjected to Smart-seq2 protocol for transcriptome analysis.