Extended Cell Panel Assay (ECPA) of swa-resistant and swa-dependent RML prions.

aPrion samples were assayed by the Standard Scrapie Cell Assay on CAD, R332H11 (2H11) and PK1 cells, the latter in the absence or presence of swainsonine (swa; 1 µg/ml) or kifunensine (kifu; 5 µg/ml). The number of PrPres positive cells per 20000 cells was plotted against the logarithm of the inocula dilutions and RIs were determined as reciprocals of the dilutions required to yield 750 PrPres positive cells per 20000 cells.bQ, the ratio of RIs (or logQ, the log of the ratios) in the absence and presence of a drug characterizes the drug sensitivity of a prion strain.cQrel = Qsample/QRML where Q = RIPK1/RIPK1+drug.