Activity parameters of folded RNase-A obtained from refolding of the Heat-, GdmCl- and Urea-denatured states in the presence of osmolytes at pH 7.0 and 25°Ca,b.
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aErrors in Km and kcat from triplicate measurements are 8–10%.bThe Units of Km and kcat are mM and min−1, respectively.*These values were obtained from enzymatic measurements after extensive dialysis for 24 hours.Activity parameters of folded RNase-A obtained from refolding of the Heat-, GdmCl- and Urea-denatured states in the presence of osmolytes at pH 7.0 and 25°Ca,b.
创建时间:
2015-12-02



