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Expression data from the HPB-ALL T-ALL cell line transduced with shETS1

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE138803
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To formally address the biological activity of ETS1 in vitro, we measured the transcriptional effect of ETS1 knock down by transducing HPB-ALL leukemia cell lines with a plKO - shETS1 and plko shLUC control. Samples were hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays. Log2 abundance estimates were obtained using gcrma package of Bioconductor, which is a version of the Robust Multi-Array Average (RMA) algorithm. We provide a supplementary file with gene annotation, which performs a two-sample T-test and computes an average fold-change. We performed microarray gene expression analysis of shETS1 vs. shRNA LUC, with 2 replicates per condition. Samples were assayed on 4 Affymetrix Human Genome U133 Plus 2.0 arrays.
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2020-10-06
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