rDNA alignment for Inocybe sect. Praetervisae

Three new species are described from eastern North America with phylogenetic alliances to members of <i>Inocybe </i>subsect. <i>Praetervisae</i> or the Praetervisa clade. All three are characterized by their relatively robust size, angular-nodulose basidiospores, entirely or nearly entirely pruinose stipe with or without a marginate bulbous base, absence of a cortina, thick-walled cystidia, and putative ectomycorrhizal associations with hardwoods such as <i>Quercus</i>, <i>Carya</i>, and/or <i>Fagus</i> in forested and urban settings. <i>Inocybe leucocaulis </i>sp. nov. is recognized from Tennessee, Indiana, and Ohio; <i>Inocybe monachella </i>sp. nov. is recorded from Indiana, Ohio, and Louisiana; and <i>Inocybe fatiscens </i>sp. nov. is recognized from Tennessee, Indiana, Pennsylvania, West Virginia, and Wisconsin. A combined phylogenetic analysis of ITS and 28S rDNA sequences supports the phylogenetic distinction of each new species with robust support. The phylogeny also recovers three internal well-supported subgroups (clades I, II, and III) ascribable to subsect. <i>Praetervisae</i>. Overall, 23 species-level lineages may belong to this subsection, but multigene analyses and further taxon sampling are needed to evaluate the monophyly of subsect. <i>Praetervisae</i>. Phylogenetic trees of combined ITS and 28S are presented, in addition to illustrations of key diagnostic features. DNA sequences of <i>rpb1 </i>and <i>rpb2 </i>gene sequences were also produced for supplemental identification and future multigene phylogenetic analyses.