Reverse genetic analysis identifies insertional mutants that disrupt pollen tube growth <i>in vitro</i>.

GUS, β-glucuronidase staining present (+) or absent (−) in pollen grains or tubes. % germ, percentage of pollen grains that germinated. aLeast squares means are reported for % germination (germ) and pollen tube length (length). n, number of pollen grains or pollen tubes analyzed. P value, statistical analysis of separation between least squares means. *P values <0.001 were considered to be statistically significant. NS, P value >0.05. 4 h PT, genes significantly higher in 4 h PT compared to dry pollen in microarray experiments. SIV PT, genes significantly higher in SIV compared to 4 h PT in microarray experiments.