Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. In our previous study, we found that iPSC-NCCs have immunosuppressive properties in resting state. In the next step, we examined gene expression patterns between iPSC-NCCs (normal condition) and iPSC-NCCs (inflamed condition). We induced NCCs from human iPSCs according to the previously described method with some modifications for 3 weeks to complete. We examined how the gene expression of iPSC-NCCs changes in inflamed condition. iPSC-NCCs were incubated with human peripheral blood mononuclear cells from 5 healthy volunteers in transwell culture plate for 5 days (inflamed condition). We extracted total RNA of iPSC-NCCs (normal condition; n=2) and iPSC-NCCs (inflamed condition; n=2).