Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression which can only be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signalling

We investigated how Interleukin 1 beta impacts equine tenocyte function and global gene expression in 3D culture and determined if these effects could be rescued by pharmacologically inhibiting nuclear factor-?B or interleukin 1 signalling. Overall design: mRNA profiles of eight independent lines of adult tenocytes were generated by RNA sequencing, using Illumina NovaSeq6000. The sequence reads that passed quality filters were analyzed using the pseudoaligner Salmon and differential expression quantified using DESeq2. Gene ontology analysis was then conducted using Panther GoSlim and pathway analysis with Enrichr.