Labeling synaptic input on individual retinal ganglion cells in non-glaucomatous retinas.
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A, Excitatory synaptic inputs were labeled by AAV2-mediated transfection of ganglion cells with PSD-95-GFP, leading to a punctuate labeling. B, To assign synaptic puncta to individual ganglion cells, the cytoskeleton of large ganglion cells was labeled with an antibody against neurofilament. C, Overlay of A and B. D, Digital representation of the dendritic arbor with assigned synaptic input sites.
创建时间:
2016-02-23




