MOESM10 of An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

Additional file 10. Size (bp) of genotyping amplicons observed for each of the six wheat homoeologs of TaNFXL1 in the T1 progeny of plants transformed with a construct expressing Cas9 and two sgRNA. To be conservative with the naming of the edited alleles, amplicons varying by less than 5 bp in size were given the same name. See Table 4 for an explication of the genotype code. N/A in amplicon size columns and ? in the genotype code indicate results not available. Genotype for Fielder, the parent line for the transgenic plants, is highlighted in grey.