Data from: Identification of stable QTL controlling multiple yield components in a durum × cultivated emmer wheat population under field and greenhouse conditions

Phentoypic data: The durum × cultivated emmer recombinant inbred line (RIL) population (referred to as DP527) was evaluated for grain yield components under greenhouse and field conditions in North Dakota, USA. The DP527 population was developed by crossing Divide (PI 642021), a North Dakota hard amber durum variety, with PI 272527, a cultivated emmer accession collected near Pest, Hungary. The DP527 population consisted of 219 RILs developed using the single-seed descent method to the F7 generation and bulked to produce F7:8 RILs. The DP527 population was evaluated under field conditions in a total of three seasons and were grown in a randomized complete block design (RCBD) with three replicates each season. Plants were grown in hill plots, with each plot consisting of 10-15 seeds and considered an experimental unit. The 2017 and 2019 plots were grown at the North Dakota State University (NDSU) field site near Prosper, ND (47.002°N, 97.115°W). The 2020 plots were grown at the NDSU agronomy seed farm near Casselton, ND (46.880°N, 97.243°W). The DP527 population and parental lines were phenotyped for 11 traits including days to heading (DTH), plant height (PHT), total number of spikelets per spike (SPS), kernels per spike (KPS), grain weight per spike (GWS), thousand kernel weight (TKW), kernel area (KA), kernel width (KW), kernel length (KL), kernel circularity (KC), and kernel length:width ratio (KLW). DTH was measured as the number of days from planting until 50% of the spikes emerged completely beyond the flag leaf. PHT was measured from the base of the hill plot to the tip of the highest spike (excluding awns) in the plot in centimeters. Eight heads from each replicate were used for phenotypic evaluations. SPS was counted as the total number of spikelets divided by the number of heads in the sample. KPS, GWS, TKW, KA, KW, KL, KC, and KLW data were obtained using a MARVIN grain analyzer (GAT Sensorik GMBH, Neubrandenburg, Germany). KPS and GWS data from the MARVIN was divided by the number of heads in the sample to obtain an average per wheat head. For the 2019 environment, planting occurred in late May, and by early September about one third of the lines were not mature. Therefore, only DTH, PHT, and SPS were evaluated in the 2019 field season. The DP527 population and parents were evaluated under greenhouse conditions in two greenhouse seasons (2018 and 2019) with two replicates per season. Plants were grown in 15 cm diameter pots in a greenhouse with 16-h photoperiod and a temperature of 21 °C. All plants were grown in a completely randomized design (CRD) with one plant per pot, which was one experimental unit. DTH was measured as the number of days from planting until the emergence of the first spike beyond the flag leaf, and PHT was measured from the base of the plant to the tip of the highest spike in centimeters. Plants were hand harvested and four heads per plant were used for the rest of the phenotypic evaluations, which were measured as described for field environments. In the data file, column headings indicate the trait evaluated, the year, field vs greenhouse, and replicate or average of all three replicates. For example, “SPS2017Frep1” indicates rep 1 of the spikelets per spike trait collected in the 2017 field trial. Sheet 1 consists of the field data, and sheet 2 is the greenhouse data. An entry of ‘NA’ indicates missing data.
Genotypic data: DNA of the DP527 population was extracted and genotyped using the Illumina iSelect 90k wheat SNP array. The genotypic data file consists of the chromosome assignments of the markers, the marker names, the linkage map positions of the markers, and the genotypic calls for each marker within each RIL where “1” represents an allele from Divide, “2” represents an allele from PI 272527, and “3” indicates missing data. This data was used to assemble the linkage-based genetic maps for the 14 durum wheat chromosomes and further used in statistical analyses to identify chromosome regions harboring genes associated with the various phenotypic traits mentioned in the phenotypic data file.
Resources in this dataset:
Resource Title: Genotypic data for the durum x emmer wheat recombinant inbred population DP527 File Name: DP527 genotypic data.xlsx Resource Description: The genotypic data file consists of the chromosome assignments of the markers, the marker names, the linkage map positions of the markers, and the genotypic calls for each marker within each RIL where “1” represents an allele from Divide, “2” represents an allele from PI 272527, and “3” indicates missing data.
Resource Title: Phenotypic data collected from the durum x emmer wheat recombinant inbred population DP527 File Name: DP527 phenotypic data.xlsx Resource Description: In the data file, column headings indicate the trait evaluated, the year, field vs greenhouse, and replicate or average of all three replicates. For example, “SPS2017Frep1” indicates rep 1 of the spikelets per spike trait collected in the 2017 field trial. Sheet 1 consists of the field data, and sheet 2 is the greenhouse data. An entry of ‘NA’ indicates missing data.

典型数据集：本研究对 durum 与栽培黑麦重组自交系（RIL）群体（以下简称 DP527）进行了评估，以探讨其在北达科他州美国温室和田间条件下的产量构成。DP527 群体由北达科他州硬 amber durum 品种 Divide（PI 642021）与在匈牙利 Pest 附近收集的栽培黑麦品种 PI 272527 杂交培育而成。该群体包含 219 个 RIL，通过单粒传代法至 F7 代并混粉得到 F7:8 RIL。DP527 群体在三个生长季节的田间条件下进行了评估，并采用随机完全区组设计（RCBD），每个季节设置三个重复。种植在埂状地块，每个地块含有 10-15 粒种子，作为一个实验单元。2017 年和 2019 年的地块种植于北达科他州立大学（NDSU）Prosper 地区的田间试验站（北纬 47.002°，西经 97.115°）。2020 年的地块种植于 NDSU 农业种子农场 Casselton 地区（北纬 46.880°，西经 97.243°）。DP527 群体及其亲本进行了 11 个性状的表型分析，包括抽穗天数（DTH）、植株高度（PHT）、每穗总小穗数（SPS）、每穗粒数（KPS）、每穗粒重（GWS）、千粒重（TKW）、籽粒面积（KA）、籽粒宽度（KW）、籽粒长度（KL）、籽粒圆形度（KC）和籽粒长宽比（KLW）。DTH 的测量是以播种至 50% 小穗完全露出旗叶的天数。PHT 的测量是从埂状地块底部至最高小穗尖端（不包括芒）的厘米数。每个重复选取八穗进行表型评估。SPS 是通过计算样本中小穗总数除以穗数得出的。KPS、GWS、TKW、KA、KW、KL、KC 和 KLW 数据使用 MARVIN 粒度分析仪（GAT Sensorik GMBH，Neubrandenburg，德国）获得。KPS 和 GWS 数据通过样本穗数进行平均。对于 2019 年的种植环境，播种发生在五月底，到九月初约三分之一的品种尚未成熟。因此，在 2019 年的田间季节中，仅评估了 DTH、PHT 和 SPS。DP527 群体及其亲本在两个温室季节（2018 年和 2019 年）的温室条件下进行了评估，每个季节设置两个重复。植物种植在直径 15 厘米的盆中，温室光照周期为 16 小时，温度为 21 °C。所有植物均采用完全随机设计（CRD），每个盆中种植一株，作为实验单元。DTH 的测量是以播种至第一个小穗露出旗叶的天数。PHT 的测量是从植株底部至最高小穗尖端的厘米数。植物手工收获，每株选取四穗进行剩余的表型评估，评估方法与田间环境相同。在数据文件中，列标题表明了评估的性状、年份、田间与温室以及重复或三个重复的平均值。例如，“SPS2017Frep1”表示在 2017 年田间试验中收集的每穗小穗数的第一重复。第一张表格包含田间数据，第二张表格包含温室数据。'NA' 的条目表示数据缺失。
基因型数据：DP527 群体的 DNA 被提取并使用 Illumina iSelect 90k 小麦单核苷酸多态性（SNP）芯片进行基因分型。基因型数据文件包括标记的染色体分配、标记名称、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用。其中，“1”代表来自 Divide 的等位基因，“2”代表来自 PI 272527 的等位基因，“3”表示缺失数据。这些数据用于构建基于连锁的遗传图谱，并进一步用于统计分析，以识别与表型数据文件中提到的各种表型性状相关的染色体区域。
本数据集中包含的资源：
资源标题：DP527 durum x emmer 小麦重组自交系群体基因型数据
文件名：DP527 基因型数据.xlsx
资源描述：基因型数据文件包含标记的染色体分配、标记名称、标记的连锁图谱位置以及每个 RIL 中每个标记的基因型调用。其中，“1”代表来自 Divide 的等位基因，“2”代表来自 PI 272527 的等位基因，“3”表示缺失数据。
资源标题：DP527 durum x emmer 小麦重组自交系群体表型数据
文件名：DP527 表型数据.xlsx
资源描述：数据文件中的列标题表明了评估的性状、年份、田间与温室以及重复或三个重复的平均值。例如，“SPS2017Frep1”表示在 2017 年田间试验中收集的每穗小穗数的第一重复。第一张表格包含田间数据，第二张表格包含温室数据。'NA' 的条目表示数据缺失。