Characterization of maize resistance to spider mite herbivores

We
surveyed a diverse collection of maize (Zea mays subsp. mays)
inbred lines for resistance to two spider mite herbivores, the generalist Tetranychus
urticae (the two-spotted spider mite) and the grass-specialist Oligonychus
pratensis (the Banks grass mite). We found that among more than 30 maize
lines, only lines B49, B75, and B96 were highly resistant to T. urticae,
and no lines were highly resistant to O. pratensis. Although our initial
screen used single strains of both mite species, in a follow up experiment, we
showed that B49, B75, and B96 are highly resistant to multiple, unrelated
strains of the T. urticae herbivore. Finally, we identified large-effect
quantitative trait loci (QTLs) on maize chromosomes one and six for resistance
to T. urticae in one or the other of these three maize lines, and we
showed that B96 was the apparent sole source of resistance alleles in our
study. The latter finding is consistent with pedigree information for B49 and
B75, which were produced previously in breeding programs that used B96.

Datasets
include phenotypic data for resistance screens of maize lines or cross-derived
populations to spider mites, as well as genomic variant data used for QTL
identification and haplotype analyses (as VCF files in an archive with a README
file describing the contents of individual files). The high-throughput genomic
sequencing reads upon which the variant data was based can be obtained publicly
from the USA National Center for Biotechnology Information (NCBI) Sequence Read
Archive (SRA) under identifiers PRJNA481365
and PRJNA556665.

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