Gene ontology of 490 iBeetle- and transcriptomics-identified genes involved in stink gland function.

The genes are listed by the Tc-number. The 249 genes recognized by the transcriptomics approaches are color coded respective to S1 Fig, with the six genes also identified in iBeetle indicated in bold (iBeetle number color code respective to Fig 3). The closest D. melanogaster homologs are denoted as indicated by iBeetle-Base [37]. In addition, gene ontology analyses were carried out using ShinyGO [44], BlastKOALA [45], and eggNOG-mapper [46]. In ShinyGO, n.a. specifies that genes were not analyzed, whereas SNARE indicates the five genes involved in “SNARE interactions in vesicular transport”. In BlastKOALA, 292 genes were not analyzed, while in eggNOG-mapper only for 125 genes no orthologs were identified. For genes screened in iBeetle, the phase of the screen is provided in the far-right column. The further analyzed gene laccase2 (Tc_010490; iB_12548) is indicated by italics and light grey background. (XLSX)