Single cell gene expression analysis of zebrafish body elongation

scRNA-seq analysis was conducted on elongating tail of developping zebrafish. We investigated the cell subpopulaiton during this developping process under purturbation of Wnt, Fgf and BMP signaling pathway. We performed single cell RNA sequencing (scRNAseq) on dissected tails from 10-12 somite stage zebrafish embryos. We used wild-type embryos and embryos subject to treatments known to alter tailbud cell migration, specifically inhibition of FGF, BMP, or Wnt signaling. For each treatment, we prepared four biological replicates each consisting of 10 to 12 tailbuds and resulting in 30,000-35,000 single cell profiles.