r<i>Ts</i>-Pmy bound to A chain of human C1q.

(A) 96-well plates were coated with different amounts of C1q (a) or 2 μg BSA (b). Increasing amounts of rTs-Pmy were added and anti-Ts-Pmy antibody 9G3 was used to detect the binding. ***p<0.001. (B) Human C1q (5 μg) was subjected to SDS-PAGE under reducing condition and stained with Coomassie blue (a). C1q and BSA (each 5 μg) under reducing condition were transferred onto a NC membrane, incubated with rTs-Pmy (5 μg/ml) and detected with anti-His mAb (1:5,000) (b). The same amount of rTs-Pmy, rTs87 or BSA (each 5 μg) were transferred to a NC membrane and then incubated with C1q (5 μg/ml). The proteins bound on the membrane were detected with anti-C1q antibody (1:1,000) (c). The rTs-Pmy on the membrane was detected with anti-His mAb (d). The consistent results are repeated for three times. M, standard protein marker.