Phenotype of tetanus toxoid (TT)- and myelin basic protein (MBP)-reactive CD3+CD8- T-cells, from untreated (UNT) and interferon (IFN)-β-treated patients with relapsing-remitting multiple sclerosis (RRMS).

CFSE-labeled PBMCs were incubated with TT and MBP for 7 days, restimulated with PMA/ ionomycin and brefeldin for 5 hours, permeabilized and stained for surface markers and intracellular cytokines with fluorochrome-conjugated antibodies. The percentage of interleukin (IL)-17A, IFN-γ, IL-4, tumor necrosis factor (TNF)-α, IL-13 and IL-10 expressing cells within CFSE-diluted (proliferating) CD3+CD8- T-cell population was assessed by flow cytometry. Data were obtained from the sub-study 2 population and are given as mean (± standard error of the mean); there were no significant differences by unpaired t-test.Phenotype of tetanus toxoid (TT)- and myelin basic protein (MBP)-reactive CD3+CD8- T-cells, from untreated (UNT) and interferon (IFN)-β-treated patients with relapsing-remitting multiple sclerosis (RRMS).