Transposon sequencing for pathway-specific unipolar polysaccharide biosynthesis screen in Agrobacterium tumefaciens C58

Performed en masse sequencing of transposon insertions among A. tumefaciens mutants deficient in unipolar polysaccharide (UPP) production in two different genetic backgrounds, each one specific for one of the two chemical species of UPP (UPPGlcN and UPPGalN) Overall design: Screening strains overproduce UPP polysaccharide due to elevated cyclic diguanylate monophosphate (cdGMP, because of deletion of pruA ATU_RS05580 regulator) and are also genetically disabled for production of multiple polysacccharides: cellulose, succinoglycan, Beta 1,2-glucans and Beta 1,3-glucans). Mutants were isolated in this back ground with either the uppY (ATU_RS02370) or the uppW (ATU_RS11495) polysaccharide polymerase genes also deleted.