The small regulatory RNA Lpr10 regulates the expression of RpoS in Legionella pneumophila

Legionella pneumophila (Lp) is a waterborne bacterium able to infect human alveolar macrophages, causing a severe pneumonia known as Legionnaires’ disease. In water, Lp grows inside ciliates and ameoba. Lp is able to survive for several months in water, while searching for host cells. In Lp, the sigma factor RpoS is important for survival in water. Several small regulatory RNAs (sRNA) are known to regulate the expression of RpoS in other bacteria. A previous transcriptomic study showed that RpoS positively regulates the sRNA Lpr10 in Lp. Microarray analysis was performed to investigate the genes regulated by Lpr10. Legionella pneumophila strains KS79 (WT), ∆lpr10, and complemented strain (∆lpr10 + plpr10) were grown in AYE broth at 37°C with shaking. When post-exponential phase was reached, RNA was extracted from triplicate samples.