Gonadotropins regulate cell adhesion and spermiation through a cross-talk between oxytocin and relaxin pathways in Senegalese sole [Dataset]

Dataset are from our own production and does not forms part of other datasets Two-year-old male Senegalese sole, F1 generation, were maintained for three months before the experiments under natural light cycles and regulated simulated natural temperatures. The experiment was conducted over six weeks from November to January to examine the specific impact of recombinant Fsh and Lh (rFsh and rLh) on testicular development. Total RNA from the testis was extracted using the RNeasy Plus Mini Kit (Qiagen). For library construction (n = 4 for each group: saline, rFsh and rLh), the SMARTseq2 protocol was employed, with some modifications. The libraries were sequenced on a NovaSeq 6000 S1 sequencing system (Illumina) in paired-end mode with 100 bp paired-end reads length. The resulting reads were processed for image analysis, base calling, and quality scoring using the manufacturer’s software, Real Time Analysis (RTA 1.18.66.3), followed by the generation of FASTQ sequence files by CASAVA 1.8.