MS proteomic datasets for "Development of methodology to investigate the surface SMALPome of mammalian cells".

In this study, mass spectrometry proteomics was used to investigate whether there are qualitative or quantitative differences in the mammalian plasma membrane proteins extracted with SMA compared to a detergent control. For this, cell surface proteins of 3T3L1 fibroblasts were biotinylated and extracted using either styrene maleic acid (SMA) or detergent. Following affinity pull-down of biotinylated proteins with NeutrAvidin beads, samples were analysed by nanoLC-MS. Here, we report for the first time, a global proteomics protocol for detection of a mammalian cell ‘SMALPome’, membrane proteins incorporated into SMA nanodiscs.