Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes following DON treatment

To identify molecular characteristics of WT AM with or without DON stimulation in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following DON treatment in WT AMs, suggesting that DON treatment altered the balance of proliferative versus inflammatory genes in AMs, and glutamine metabolism is important in regulating AM self-renewal ability. Comparative gene expression profiling analysis of RNA-seq data for AM following DON treatment