Sensing of extracellular L-Proline availability by the integrated stress response determines the outcome of cell competition

To address the mechanism by which ATF4 determines the outcome of cell
competition, we performed transcriptomic comparisons by bulk
RNA-seq analysis of up-regulated, down-regulated and background
genes related to the DE analysis of homogenous cultures of Atf4-/- versus
wild-type mouse ES cells and of p53-/-; Atf4-/- versus
p53-/- mouse ES cells at day 3 of differentiation. Differential
cellular pathway analysis by KEGG enrichment revealed that the topmost
downregulated pathways in both sets of cells with Atf4 mutations were
those associated with non-essential amino acid biosynthesis, transport and
metabolism