RNA-seq study of mycolic acid deficiency in Corynebacterium glutamicum ATCC13869

We constructed mycolic acid synthesis key gene pks13 mutant strain and analyzed its impacts on whole cell at gene expression level compared to the wild-type ATCC13869. The Corynebacterium glutamicum ATCC13869 in-frame deletion pks13 mutant (renamed WG001) and wild-type ATCC13869 were analyzed by RNA-seq. All samples were cultivated in BHI medium until reaching exponential phase. Cultures were pooled for RNA extraction, library preparation and sequencing. qRT–PCR validation was performed using SYBR Green assays.