RNA-seq data from MDA-MB-231 cells. RNA-seq data from MDA-MB-231 cells

To understand the role of GPR81 in cancaer malignancy, we generated silenced GPR81 using shRNA in MDA-MB-231 cells and performed RNA-seq analysis. Overall design: Total RNA was isolated from shNT MDA-MB-231 cells and shGPR81 MDA-MB-231 cells. Total RNA library preparation was performed using a TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA), in accordance with the manufacturer’s protocol. Sequencing was performed on an Illumina HiSeq 2500 platform in 75-base single-end mode. Illumina Casava1.8.2 software was used for base-calling. Sequenced reads were mapped to the mouse reference genome sequence (mm10) using TopHat v2.0.13 in combination with Bowtie2 ver. 2.2.3 and SAMtools ver. 0.1.19.